| Ayanami Rei | Hayashibara Megumi |
| Ikari Shinji | Ogata Megumi |
| Souryuu Asuka Langley | Miyamura Yuko |
| Katsuragi Misato | Mitsuishi Kotono |
| Akagi Ritsuko | Yamaguchi Yuriko |
| Ikari Gendou | Tachiki Fumihiko |
| Fuyutsuki Kouzou | Kiyokawa Motomu |
| Ibuki Maya | Nagasawa Miki |
| Hyuuga Makoto | Yuuki Hiro |
| Aoba Shigeru | Koyasu Takehito |
| Kaji Ryouji | Yamadera Kouichi |
| Keel Lorentz | Mugihito |
| Announcer (ep.23) | Nagasako Takashi |
| Technical Department Personnel (ep.23) | Iwanaga Tetsuya |
| SEELE Member <Credited as "Committee Member"> | Ishino Ryuuzou |
| SEELE Member (Uncredited) | Koyasu Takehito |
| SEELE Member (Uncredited) | Nagasako Takashi |
| SEELE Member (Uncredited) | Iwanaga Tetsuya |
| Pen Pen (Uncredited) | Hayashibara Megumi |
