| Penguin Musume - Heart (2008/04/19-2008/11/25 Internet Anime)
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| Nankyoku Sakura/Penguin | Kataoka Azusa |
| Etorofu Kujira | Ise Mariya |
| Kurio Nene | Nanjou Yoshino |
| Shaa Chii | Nogawa Sakura |
| Sebasuchan | Ishii Kouji |
| Nankyoku Kaede | Hidaka Rina |
| Marii Chupakabura W Howaiyobea | Kadowaki Mai |
| Meari Chupakabura W Howaiyobea | Shiono Anri |
| Kodama Kiyomi | Akesaka Satomi |
| Ijuuin Karei | Suzuki Yuuto |
| Hoojiro Maguro | Suzuki Masami |
| Hoojiro Saki | Miruno Jun |
| Mariana | Honda Chieko |
| Riifu | Akiya Tomoko |
| Akua | Shishido Rumi |
| Iruka-sama | Tomatsu Haruka |
| Konsaba Makoto | Nakamura Daisuke |
| Ebi-sensei | Namiki Noriko |
| Kujira's Father | Matsuyama Takashi |