| Shimura Shinpachi | Sakaguchi Daisuke |
| Kagura | Kugimiya Rie |
| Sakata Gintoki | Sugita Tomokazu |
| Katsura Koutarou | Ishida Akira |
| Hijikata Toushirou | Nakai Kazuya |
| Okita Sougou | Suzumura Kenichi |
| Harada | Miyake Kenta |
| Otose | Kujira |
| Sadaharu | Takahashi Mikako |
| Otae | Yukino Satsuki |
| Kondou Isao | Chiba Susumu |
| Catherine | Sugimoto Yuu |
| ???? | Chafuurin |
| Kurogoma Katsuo | Ishizuka Katashi |
| Chin Pirako/Doromizu Pirako | Nonaka Ai |
| Hasegawa Taizou | Tachiki Fumihiko |
| Old Man (ep.11-12) | Shimada Bin |
| Jouji (ep.16) | Chiba Isshin |
| Gengai (ep.17) | Aono Takeshi |
| ???? (ep.18) | Sonobe Keiichi |
| Ebina (ep.21) | Ueda Yuuji |
| Girl (ep.21) | Shitaya Noriko |
| Santa (ep.35) | Ogata Kenichi |
| Hanako (ep.36) | Ueda Kana |
| Kada (ep.49) | Itou Miki |
| Kyoushirou (ep.55) | Kisaichi Atsushi |
| Tsunpo (ep.56) | Yamazaki Takumi |
| Kawakami Bansai (ep.58) | Yamazaki Takumi |
| Okuni (ep.63) | Shimizu Ai |
| Otose (young) | Enomoto Atsuko |
| Doushin | Nakata Jouji |
| Otsuu | Takahashi Mikako |
| Daraku | Nakao Ryuusei |
| Yocchan | Yukino Satsuki |
| Takasugi | Koyasu Takehito |
| Sarutobi Ayame | Kobayashi Yuu |
| Girl | Sugimoto Yuu |
| 松子 (ep.110) | Nakamura Eriko |
| Roushi A [Lordless Samurai A] | Imai Akinobu |
| Matsuko | Nishikawa Hazuki |
| Prostitute B | Yazawa Kiyomi |
| Hata Ouji | Sakaguchi Kouichi |
| Musashippoi Hito [Musashi-like Person] | Sakaguchi Kouichi |
| Tamo-san | Sakaguchi Kouichi |
| Shinpachi's Father (Shimura Ken) | Sakaguchi Kouichi |
| Shishimura | Sakaguchi Kouichi |
| Jai Jen | Sakaguchi Kouichi |
| Kinya | Sakaguchi Kouichi |
| Nojima | Sakaguchi Kouichi |
| Training Institute Professor | Sakaguchi Kouichi |
| 角刈-oyabun | Sakaguchi Kouichi |
| Tenjin [Celestial Being] | Sakaguchi Kouichi |
| Nobunaga | Sakaguchi Kouichi |
| Gunsou [Sergeant] | Sakaguchi Kouichi |
| Game-sennin | Sakaguchi Kouichi |
| Stand-in Takasugi | Sakaguchi Kouichi |
| Sakamoto Tatsuma | Miki Shinichirou |
| Watanabe Tokunosuke | Miki Shinichirou |
| Hanano Announcer | Tamura Yukari |
| Maguko | Makiguchi Mayuki |
